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precision plus westernc standards ladder  (Bio-Rad)


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    Bio-Rad precision plus westernc standards ladder
    Precision Plus Westernc Standards Ladder, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/precision+plus+protein+westernc+blotting+standard/Precision+Plus+Protein+WesternC+Blotting+Standards/bio_rxiv__64898__2026__04__24__720702-307-17-22
    Average 99 stars, based on 577 article reviews
    precision plus westernc standards ladder - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Molecular Weight:

    Article Title: Proteomic and Lipidomic Profiling of Immune Cell-Derived Subpopulations of Extracellular Vesicles.
    Article Snippet: The samples were denatured at 95◦C for 5 min before loading onto Mini-Protean TGX precast 4%– 20% gels (Bio-Rad Laboratories). .. As a molecular weight marker, 4 μL Precision Plus Protein WesternC Blotting Standard (Bio-Rad Laboratories) was used. .. The proteins were separated at 180 V for about 45 min. Blotting onto PVDF membranes was performed on the Trans-Blot Turbo Transfer system (Bio-Rad Laboratories) set to 1.3 A and 25 V for 7 min using transfer buffer [100 mL TransBlot Turbo stock solution (Bio-Rad Laboratories), 100 mL ethanol and 300 mL distilled water].

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model.
    Article Snippet: .. Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anticlaudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS)+ 0.2% Tween-20 at 4 °C, overnight on a shaker.

    Article Title: Female Type 1 Diabetic Akita Mice Demonstrate Increased Bladder Contractility via FP Receptor Activation due to NLRP3-Mediated Inflammation.
    Article Snippet: Reduced proteins were then loaded into two separate 4–12%Bis-Tris SDS polyacrylamide gels (Invitrogen, Waltham, MA, USA) with each gel containing 50 μg of protein per detrusor. .. Both gels containing the molecular weight marker, Precision Plus Protein WesternC Blotting Standard (Bio-Rad; Hercules, CA, USA), underwent simultaneous electrophoresis in a duel chamber tank containing 1×MES SDS Running buffer (NuPage; Invitrogen) at a constant 80V for 2.5 hours. .. Both gels were then transferred onto the same nitrocellulose membrane (0.2 μm pore size) using the iBlot 2 semi-dry transfer apparatus (8 minutes, 20V; Invitrogen).

    Article Title: Immunoassays for the detection and differentiation of Paenibacillus larvae, the etiological agent of American foulbrood (AFB) in honey bees.
    Article Snippet: .. The Precision Plus Protein WesternC Blotting Standard (Bio-Rad, Feldkirchen, Germany) served as a molecular weight marker for all blots. ..

    Article Title: Proteomic and Lipidomic Profiling of Immune Cell‐Derived Subpopulations of Extracellular Vesicles
    Article Snippet: The samples were denatured at 95°C for 5 min before loading onto Mini‐Protean TGX precast 4%–20% gels (Bio‐Rad Laboratories). .. As a molecular weight marker, 4 μL Precision Plus Protein WesternC Blotting Standard (Bio‐Rad Laboratories) was used. .. The proteins were separated at 180 V for about 45 min. Blotting onto PVDF membranes was performed on the Trans‐Blot Turbo Transfer system (Bio‐Rad Laboratories) set to 1.3 A and 25 V for 7 min using transfer buffer [100 mL Trans‐Blot Turbo stock solution (Bio‐Rad Laboratories), 100 mL ethanol and 300 mL distilled water].

    Article Title: Immunoassays for the detection and differentiation of Paenibacillus larvae , the etiological agent of American foulbrood (AFB) in honey bees
    Article Snippet: .. The Precision Plus Protein WesternC Blotting Standard (Bio-Rad, Feldkirchen, Germany) served as a molecular weight marker for all blots. ..

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model
    Article Snippet: .. Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20 mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anti-claudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS) + 0.2% Tween-20 at 4 °C, overnight on a shaker.

    Marker:

    Article Title: Proteomic and Lipidomic Profiling of Immune Cell-Derived Subpopulations of Extracellular Vesicles.
    Article Snippet: The samples were denatured at 95◦C for 5 min before loading onto Mini-Protean TGX precast 4%– 20% gels (Bio-Rad Laboratories). .. As a molecular weight marker, 4 μL Precision Plus Protein WesternC Blotting Standard (Bio-Rad Laboratories) was used. .. The proteins were separated at 180 V for about 45 min. Blotting onto PVDF membranes was performed on the Trans-Blot Turbo Transfer system (Bio-Rad Laboratories) set to 1.3 A and 25 V for 7 min using transfer buffer [100 mL TransBlot Turbo stock solution (Bio-Rad Laboratories), 100 mL ethanol and 300 mL distilled water].

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model.
    Article Snippet: .. Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anticlaudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS)+ 0.2% Tween-20 at 4 °C, overnight on a shaker.

    Article Title: Female Type 1 Diabetic Akita Mice Demonstrate Increased Bladder Contractility via FP Receptor Activation due to NLRP3-Mediated Inflammation.
    Article Snippet: Reduced proteins were then loaded into two separate 4–12%Bis-Tris SDS polyacrylamide gels (Invitrogen, Waltham, MA, USA) with each gel containing 50 μg of protein per detrusor. .. Both gels containing the molecular weight marker, Precision Plus Protein WesternC Blotting Standard (Bio-Rad; Hercules, CA, USA), underwent simultaneous electrophoresis in a duel chamber tank containing 1×MES SDS Running buffer (NuPage; Invitrogen) at a constant 80V for 2.5 hours. .. Both gels were then transferred onto the same nitrocellulose membrane (0.2 μm pore size) using the iBlot 2 semi-dry transfer apparatus (8 minutes, 20V; Invitrogen).

    Article Title: Immunoassays for the detection and differentiation of Paenibacillus larvae, the etiological agent of American foulbrood (AFB) in honey bees.
    Article Snippet: .. The Precision Plus Protein WesternC Blotting Standard (Bio-Rad, Feldkirchen, Germany) served as a molecular weight marker for all blots. ..

    Article Title: Proteomic and Lipidomic Profiling of Immune Cell‐Derived Subpopulations of Extracellular Vesicles
    Article Snippet: The samples were denatured at 95°C for 5 min before loading onto Mini‐Protean TGX precast 4%–20% gels (Bio‐Rad Laboratories). .. As a molecular weight marker, 4 μL Precision Plus Protein WesternC Blotting Standard (Bio‐Rad Laboratories) was used. .. The proteins were separated at 180 V for about 45 min. Blotting onto PVDF membranes was performed on the Trans‐Blot Turbo Transfer system (Bio‐Rad Laboratories) set to 1.3 A and 25 V for 7 min using transfer buffer [100 mL Trans‐Blot Turbo stock solution (Bio‐Rad Laboratories), 100 mL ethanol and 300 mL distilled water].

    Article Title: Immunoassays for the detection and differentiation of Paenibacillus larvae , the etiological agent of American foulbrood (AFB) in honey bees
    Article Snippet: .. The Precision Plus Protein WesternC Blotting Standard (Bio-Rad, Feldkirchen, Germany) served as a molecular weight marker for all blots. ..

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model
    Article Snippet: .. Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20 mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anti-claudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS) + 0.2% Tween-20 at 4 °C, overnight on a shaker.

    BIA-KA:

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model.
    Article Snippet: .. Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anticlaudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS)+ 0.2% Tween-20 at 4 °C, overnight on a shaker.

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model
    Article Snippet: .. Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20 mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anti-claudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS) + 0.2% Tween-20 at 4 °C, overnight on a shaker.

    Electrophoresis:

    Article Title: Female Type 1 Diabetic Akita Mice Demonstrate Increased Bladder Contractility via FP Receptor Activation due to NLRP3-Mediated Inflammation.
    Article Snippet: Reduced proteins were then loaded into two separate 4–12%Bis-Tris SDS polyacrylamide gels (Invitrogen, Waltham, MA, USA) with each gel containing 50 μg of protein per detrusor. .. Both gels containing the molecular weight marker, Precision Plus Protein WesternC Blotting Standard (Bio-Rad; Hercules, CA, USA), underwent simultaneous electrophoresis in a duel chamber tank containing 1×MES SDS Running buffer (NuPage; Invitrogen) at a constant 80V for 2.5 hours. .. Both gels were then transferred onto the same nitrocellulose membrane (0.2 μm pore size) using the iBlot 2 semi-dry transfer apparatus (8 minutes, 20V; Invitrogen).



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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: Early-stage multi-cancer detection through a plasma extracellular vesicle protein signature

    doi: 10.1016/j.xcrm.2026.102694

    Figure Lengend Snippet:

    Article Snippet: Precision Plus ProteinTM WesternCTM Blotting Standards , Bio-Rad , Cat# 1610376.

    Techniques: Recombinant, Modification, Western Blot, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Mass Spectrometry, Software